题目

基因克隆是对分离出来的目的基因进行大量扩增的过程.经过基因克隆,可以生产出大量的目的基因或在基因表达时获得大量的蛋白质.细菌质粒是基因克隆的合适载体,经研究发现,某些细菌质粒中含有两个抗生素抗性基因﹣﹣氨苄青霉素抗性基因和四环素抗性基因,而且,在四环素抗性基因中存在限制酶的单一识别序列.如图表示利用质粒进行基因克隆的过程,其中A﹣D表示物质,I和II表示抗性基因,①﹣⑦表示过程,其它数字表示菌落,据图回 阅读理解   In spite of the uncertainty of the economy, the movie industry has been stricken by a box-office outburst.Suddenly it seems as if everyone is going to the movies, with ticket sales this year up 17.5 percent, to $1.7 billion.   And it is not just because ticket prices are higher.Attendance has also jumped, by nearly 16 percent.If that pace continues through the year, it would amount to the biggest box-office increase in at least two decades.   Americans, for the moment, just want to hide in a very dark place.People want to forget their troubles, and they want to be with other people.Helping feed the outburst is the mix of movies, which have been more audience-friendly in recent months as the studios have tried to adjust after the discouraging sales of more serious films.   As she stood in line at the 18-screen Bridge theater complex here on Thursday to buy weekend tickets for “Jonas Brothers:The 3D Concert Experience,” Angel Hernandez was not thinking much about escaping reality.Instead, Ms.Hernandez, a Los Angeles parking lot attendant and mother of four young girls, was focused on one very specific reality:her wallet.   “Spending hundreds of dollars to take them to Disneyland is ridiculous right now,” she said.“For $60 and some candy money I can still be a good mom and give them a little fun.”   A lot of parents may have been thinking the same thing Friday, as “Jonas Brothers” sold out more than 800 theaters, and was expected to sell a powerful $25 million or more in tickets.   The film industry appears to have had a hand in its recent good luck.Over the last year or two, studios have released movies that are happier, scarier or just less depressing than what came before.After poor results for a rush of serious dramas built around the Middle East, Hollywood got back to comedies. (1) Which of the following is not a reason for the improvement of the movie industry? [  ] A. A growing number of people are going to the cinema. B. People are richer with the development of economy. C. More comedies are made than serious films. D. People have to pay more to watch a movie. (2) Ms.Hernandez purchased the movie tickets because ________. [  ] A. she tried to escape reality B. she was a crazy movie fan C. she was fond of Disneyland D. she wanted to please her kids (3) According to the text, which of the following number is not used to describe the shooting up of the movie industry? [  ] A. 17.5% B. $1.7 billion C. $60 D. $25 million (4) The passage is developed mainly by ________. [  ] A. presenting the effect and analyzing the causes B. following the order of time C. describing problems and drawing a conclusion D. making comparison of ideas
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