题目

Cody and his sister April went into a local pet store. It was a very small pet store that didn’t have many animals. The owner of the shop, Mr. Smith, greeted  Cody and April when  they   36   .April said, “We would like to   37   a dog.” “Ah, well, our shop is not very    38    ，” Mr Smith told her. “So we only have two dogs to choose from.”    They asked Mr. Smith to    39     them the dogs. Mr. Smith took them to see the two dogs. One was a big Bulldog named Buster. The other was a tiny Chihuahua named Teacup. April wanted Teacup, but Cody wa16．2009年10月，我国自主研发的转基因抗虫水稻“华恢1号”获得农业部颁发的安全证书．如图1表示该抗虫水稻主要培育流程，据图回答：（1）④过程应用的主要生物技术是植物组织培养．（2）杀虫基因（crylA）是人们根据几种Bt毒蛋白的分子结构，设计并人工合成的，这属于蛋白质工程技术范畴．（3）组建理想的载体需要对天然的质粒进行改造．如图2是天然土壤农杆菌Ti质粒结构示意图（示部分基因及部分限制性内切酶作用位点），据图分析：①人工改造时，要使抗虫基因表达，还应插入启动子．②人工改造时用限制酶Ⅱ处理，其目的是：第一，去除质粒上的tms和tmr（基因），保证T-DNA进入水稻细胞后不会引起细胞的无限分裂和生长；第二，使质粒带有单一限制酶作用位点，有利于目的基因（或外源DNA）准确插入．第三，使质粒大小合适，可以提高转化效率等．③若用限制酶Ⅰ分别切割改造过的理想质粒和带有抗虫基因的DNA分子，并构成重组Ti质粒．分别以含四环素和卡那霉素的培养基培养已成功导入抗虫基因的水稻胚细胞，观察到的细胞生长的现象是在含卡那霉素的培养基能够生长，而在含四环素的培养基中不能生长．（4）若限制酶Ⅱ切割DNA分子后形成的粘性末端为$\left.\begin{array}{l}{-CTGCA}\\{-G}\end{array}\right.$，则该酶识别的核苷酸序列是GACGTC．